Glomerular changes in microscopic haematuria, studied by quantitative immunoelectron microscopy and in situ zymography

Glomerular changes in microscopic haematuria, studied by quantitative immunoelectron microscopy and in situ zymography.

BACKGROUND Haematuria of glomerular origin, even if mild, implies the development of defects in the glomerular basement membrane (GBM). In diseases where there is no infiltration of leukocytes into the glomerulus-such as thin basement membrane disease (TBMD) and histologically mild cases of IgA nephropathy (IgAN)-the mechanism by which such defects form is unclear. METHODS Frozen renal tissue...

Differentiation of Glomerular from Non-Glomerular Hematuria by Three Different Me-thods of Microscopic Examinations of Erythrocytes in Urine

Background: Morphological examinations of urinary erythrocytes can be of diagnostic value in initial evaluation of hematuria. Dysmorphic urinary red blood cells are known to indicate a glomerular origin of bleeding. We examined the clinical usefulness of this test in a population complained of hematuria by use of three different methods: light microscopy, phase contrast microscopy, and Wright s...

Painless Microscopic Haematuria

In situ zymography : topographical considerations * Bruce

In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the a...

Painless Microscopic Haematuria